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FnCas12a, Glycerol-Free

Recombinant His-tagged Francisella tularensis protein expressed in E. coli. Enzyme supplied with 1 mL of Cas12 reaction buffer, 10x (CS12-09).

CF12A-E311HB
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*The image is illustrative and does not necessarily represent the product


Alternate Names:

CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1 1, FnCas12a 1, FnCpf1


UniProt Number:

A0Q7Q2


配方:

Protein stored in 25 mM Tris, 300 mM NaCl, pH7.5, 0.1 mM TCEP


参考文献:

1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. Mohanraju P, et al: Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles. CRISPR J. Feb;4(1):82-91. 2021.

存储稳定性:

Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.


浓度:

1.0 ?g/?l
6.53 pmol/?g


Molecular Weight:

Calculated: 153.2 kDa
Observed: ~130 kDa


纯度:

>90%


Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.




There are no related publications available for this product.