TtCsm6, Active

概述:

TtCsm6 is a single-stranded RNA-specific endoribonuclease protein that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer and it contributes to the Type-III-A CRISPR-Cas system (1). Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex (2).

Accession 编号:

WP_011229148.

配方:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.

参考文献:

1. Niewoehner, O. et al: Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. Rna 22(3):318-329,2016.

2. Niewoehner, O. et al: Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548, 2017.

存储稳定性:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Specific Activity:

Sample activity data. for lot-specific data, please refer to the lot-specific datasheet.

纯度:

Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.

分子量:

MW 53kDa