SpCas9, Active

概述:

Streptococcus pyogenes Cas9 is a type II-A Cas9 protein and is the most used in most current CRISPR-based genetic engineering methodologies (1). CRIPSR RNAs (crRNAs) fused to trans-activating CRISPR RNAs (tracrRNA) direct sequence-specific cleavage by Cas9, introducing double-stranded breaks in cell-invading DNA in bacterial and archaeal CRIPSR/Cas9 systems (1,2). Cas9 requires the 5’-NGG-3’ protospacer adjacent motif (PAM) to destabilize the target DNA (3). Cas9’s HNH endonuclease domain cleaves the strand complementary to the crRNA while its RuvC endonuclease domain cleaves the non-complementary strand (2).

Accession 编号:

AAK33936.

配方:

Recombinant protein stored in 50mM sodium phosphate, pH 8.0, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.

参考文献:

1. Jinek, M. et al. Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation. Science. 2014, 343(6176): 1247997.

2. Jinek, M. et al. A programmable dual RNA-guided endonuclease in adaptive bacterial immunity. Science. 2012, 337(6096): 816-821.

3. Anders, C. et al. Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease. Nature. 2014, 513(7519): 569-573.

存储稳定性:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Specific Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet

纯度:

Sample Purity graph. For specific information on a given lot, see related technical data sheet

Molecular Weight:

Approx 156kDa