TccCas13a, Active

概述:

CRISPR-Cas systems constitute the adaptive immune system in bacteria via RNA-guided endonucleases (1). Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas system (2). Cas13a possess both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (3). Thermoclostridium caenicola Cas13a is thermostable and exhibits robust cis and trans catalytic activities over a broad range of temperatures from 37 to 70 °C (4). This enzyme may be used for various biotechnological applications including diagnostics development, transcriptome engineering, editing, and targeted therapeutics.

Accession 编号:

WP_149678719.

配方:

Recombinant protein stored in 150mM imidazole, 50mM sodium phosphate pH 7.5, 10% glycerol, 1mM DTT.

参考文献:

1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.

2. East-Seletsky A, et al. 2016. Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 538:270–3.

3. Abudayyeh O, et al. 2016. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 353:5573.

4. Mahas A, et al. 2022. Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2. Proc Natl Acad Sci U S A. 119(28):e2118260119.

存储稳定性:

Store product at –70oC. For optimal performance aliquot product into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated handling and multiple freeze/thaw cycles. Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions.

Alternative Name(s):

Type VI-A CRISPR-associated RNA-guided ribonuclease Cas13a

Specific Activity:

Sample Activity Data. For specific information on a given lot, see related technical data sheet.

纯度:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Molecular Weight:

Observed MW ~130 kDa Calculated MW 144.7 kDa