LwCas13a, Active

概述:

Cas13a is RNA-guided RNA endonuclease that belongs to class 2 type VI CRISPR-Cas system (1). Cas13a possess both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. This property of Cas13a enzyme has been leveraged for the development of sensitive and robust nucleic acid detection diagnostic tool for clinical samples. CRISPR-Cas13a system provides opportunity for the development of clinical research in precision oncology (3).

Accession 编号:

WP_021746774.

配方:

Recombinant protein stored in 50mM sodium phosphate, pH 8.0, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.

参考文献:

1. East-Seletsky A, et al: Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016, 538:270–3.

2. Abudayyeh O, et al: C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016, 353:5573.

3. Patchsung, M, et al: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020. https://doi.org/10.1038/s41551-020-00603-x.

4. Zhang J, You Y. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 2020, 17(1):6-8.

活性:

Sample activity data. For lot-specific data, please refer to the specific datasheet.

Purity :

Sample purity data. For lot-specific data, please refer to specific datasheets.

Alternative Name (s):

CRISPR-associated endoribonuclease Cas13a, LwC2c2, LwaCas13a, EndoRNase, Uniprot accession U2PWF1.

Molecular Weight:

Approx 130 kDa

Storage, Stability, and Shipping:

Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions.