LbuCas13a, Active

概述:

Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas system (1). Cas13a possesses both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). Engineered LbuCas13a shows stability and altered cleavage activity in vitro and possesses the capability of robust exon skipping in human cells (4).

Accession 编号:

WP_015770004

配方:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 1mM DTT, 10% glycerol.

参考文献:

1. East-Seletsky A, et al: Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016, 538:270–3.

2. Abudayyeh O, et al: C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016, 353:5573.

3. Patchsung M, et al: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020. https://doi.org/10.1038/s41551-020-00603-x.

4. Charles E, et al: Engineering improved Cas13 effectors for targeted post-transcriptional regulation of gene expression. bioRxiv. doi: https://doi.org/10.1101/2021.05.26.445687.

存储稳定性:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

纯度:

Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.

分子量:

132kDa

Specific Activity:

Sample activity data. for lot-specific data, please refer to the lot-specific datasheet