主页   >   IVD原料   >    CRISPR    >    Cas 酶

Signalchem Product Image

LbuCas13a, Glycerol-Free

Recombinant full-length, tag-free Leptotrichia buccalis protein LbuCas13a expressed in E. coli. Enzyme supplied with 1 mL of Cas13 reaction buffer II, 10x (CS13-09B).

CS13A-E322UB
50 ug100 ug Bulk Customize

*The image is illustrative and does not necessarily represent the product


Alternate Names:

CRISPR-associated endoribonuclease C2c2, EndoRNase, LbuC2c2, c2c2, Cas13a


UniProt Number:

C7NBY4


配方:

Protein stored in 25 mM Tris, pH 7.5, 300 mM NaCl, 0.1 mM TCEP


参考文献:

1. East-Seletsky A, et al. 2016. Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 538:270–3.
2. Abudayyeh O, et al. 2016. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 353:5573.
3. Zhang J, You Y. 2020. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 17(1):6-8.
4. Patchsung M, et al. 2020. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 4(12):1140-1149.

浓度:

1.0 ?g/?l
7.14 pmol/?g


Molecular Weight:

Calculated: 138.5 kDa
Observed: ~120 kDa


纯度:

>95%


Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.


Storage and Shipping:

Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot diluted product into smaller quantities.




There are no related publications available for this product.