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CcaCas13b

Recombinant Capnocytophaga canimorsus CcaCas13b protein with a His tag expressed in E. coli. Cas13 enzyme is sold with 1ml of Cas13 reaction buffer II, 10X (CS13-09B).

CS13B-E311H
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*The image is illustrative and does not necessarily represent the product


Alternate Names:

Type VI-B CRISPR-associated RNA-guided ribonuclease Cas13b, Cas13b


UniProt Number:



配方:

Protein stored in 50 mM Tris, pH 7.5, 600 mM NaCl, 0.1 mM TCEP, and 50% glycerol


参考文献:

1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Smargon, A. A., et al. 2017. Cas13b is a Type VI-B CRISPR-associated RNA-Guided RNAse differentially regulated by accessory proteins Csx27 and Csx28. Mol. Cell. 65(4):618-630.
4. Freije, C. A., et al. 2019. Programmable Inhibition and Detection of RNA Viruses Using Cas13. Mol. Cell. 76(5):826-837.

存储稳定性:

Store at –20°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot product into smaller quantities.


浓度:

1.0 ?g/?l
6.9 pmol/?g


Molecular Weight:

Calculated: 144.7 kDa
Observed: ~130 kDa


纯度:

>90%


Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.




There are no related publications available for this product.