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LsCsm6

Recombinant Ligilactobacillus salivarius protein LsCsm6 was expressed in E. coli cells using a His tag.

LS01-E31H
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Alternate Names:

CRISPR system endoribronuclease Csm6, CRISPR type III-A associated protein Csm6


UniProt Number:



配方:

Protein stored in 25 mM Tris, 300 mM NaCl, pH 7.5, 0.1 mM TCEP, 50% glycerol


参考文献:

1. Niewoehner, O. et al. 2016. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22(3):318-329.
2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.

存储稳定性:

Store at –20°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.


浓度:

1.0 ?g/?l
20.4 pmol/?g


Molecular Weight:

Calculated: 49.6 kDa
Observed: ~49 kDa


纯度:

The purity was determined to be >90% by densitometry


Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.




There are no related publications available for this product.