Taq Plus DNA polymerase

概述:

The fidelity of Taq Plus is 6 times greater than that of Taq DNA Polymerase. Taq Plus also has stronger amplification performance, higher sensitivity and yield and is more tolerant of impurities within 5 kb amplifying range, compared to Taq DNA Polymerase. The resulting PCR products contain A at the 3’-end and can be directly cloned into T-Vectors

Applications of this product include routine PCR amplification of DNA fragments, generation of PCR products for TA cloning, DNA labelling and DNA sequencing.

配方:

10 mM Tris-HCl, pH 7.4, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% IGEPAL CA-630, 50% glycerol

参考文献:

1. Innis M A, et al: PCR protocols and applications: A laboratory manual, academic, New York, 1989.
2. Innis M A, et al: DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA, Proc. Natl. Acad. Sci. USA. 1988, 85:9436-9440.
3. Weyant R S, et al: Effect of ionic and nonionic detergents on the Taq polymerase. Biotechniques. 1990, 9:308-309.

存储稳定性:

Store product at –20oC. For optimal performance aliquot product into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated handling and multiple freeze/thaw cycles.

Components:

Component Name
Taq Plus DNA polymerase (5U/µl)
10X Taq Plus Buffer (Mg2+ plus)

Quality Control:

Exonuclease Activity: The product is tested in a reaction containing 10 U of Taq Plus DNA Polymerase and 0.6 g of λ-Hind III DNA. After incubation at 37oC for 16 hours, there is no visually detectable change of pattern of DNA bands determined by agarose gel electrophoresis.

Endonuclease Activity: The product is tested in a reaction containing 10 U of Taq Plus DNA Polymerase and 0.6 g of supercoiled pBR322. After incubation at 37oC for 4h, there is no visually detectable change of pattern of DNA bands determined by agarose gel electrophoresis.

Functional Assay: The human -1-antitrypsin gene is amplified for 30 cycles in a 50 l system using 1.25 U of Taq Plus DNA Polymerase and 100 ng of human genomic DNA as template. A single DNA band of 360 bp is detected by 1% agarose gel electrophoresis.

Specific Activity:

The specific activity of Taq Plus DNA Polymerase was determined to be 5 Units/µl.
Unit Definition:
One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as template/primer.