AapCas12b, Active

概述:

CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). AapCas12b belongs to the type V CRISPR effector, CRISPR-Cas12b/C2c1, and due to its thermostability, it can be used in a wide range of biomedical applications including loop-mediated isothermal amplification (LAMP), mammalian genome editing, and gene activation (4).

Accession 编号:

WP_067623834.1

配方:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 1mM DTT, 10% glycerol.

参考文献:

1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Lucia C, et al: An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12. bioRxiv. doi: https://doi.org/10.1101/2020.02.29.971127.

3. Teng F, et al: Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell discovery. 2018, 4(1):1-5.

存储稳定性:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles

纯度:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.

分子量:

128kDa

Specific Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet.