AsCas12a, Active

概述:

CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). AsCas12a is a CRISPR-associated endonuclease from Acidaminococcus and it can be engineered to achieve combinatorial genetic screening with high efficiency (2). Engineered AsCas12a further enables accurate editing in hematopoietic stem cells, induced pluripotent stem (iPS) cells, and natural killer (NK) cells and helps fight against sickle cell disease (4).

Accession 编号:

WP_021736722.

配方:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT and 10% glycerol.

参考文献:

1. Horvath, P., et al. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.

2. Morange, M. What history tells us XXXVII. CRISPR-Cas: The disconvery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.

3. Gier, R.A., et al. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 2020, 11(1):1-9. 4. DeLong, R. Ushering in a new era of RNA-based therapies. Commun Biol. 2021. doi: https://doi.org/10.1038/s42003-021-02150-w

存储稳定性:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

活性:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet

纯度:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Molecular Weight:

130 kDa